Lipases (triacylglycerol acylhydrolases, EC.18.104.22.168) are important enzymes due to their various industrial applications. The ability of lipases to catalyze both hydrolytic and synthetic reactions has directed research towards seeking better understanding of their properties as well as their potential applications. Despite the great industrial potential of lipases, the high production cost of lipases limits their industrial applications. Recently, lipases have been produced using microbial sources along with agro-industrial wastes to reduce the production cost especially employing solid-state bio-conversion for the process. Based on that, I was working on Palm kernel cake (PKC) as one of the solid wastes obtained after oil extraction from palm kernel seeds. The nutritive value of PKC directed studies towards utilization of this substrate in order to produce enzymes as it is a rich source of carbohydrates as well as protein and minerals which makes it suitable for microbial fermentation. All pictures provided are the author's copyright and taken during the experimental sessions.
Ionic liquid-Compatible Cellulase
The pesearch involves bioethanol production with details on the production of cellulase enzyme from Trichoderma reesei (RUTC30) from agricultural residues and lignocellulosic biomass and conducting the synthesis of the ionic liquids from their raw materials. The aim is to find a suitable and compatible ionic liquid that can stabilize the cellulase enzyme to conduct a single-step hydrolysis followed by fermentation of the hydrolysate using yeast.
Trichoderma reesei ;
Trichoderma reesei (microscopic image); 5-day culture
Solid-state fermentation of rice husk by Trichoderma reesei
Ionic Liquid Synthesis
Biomass Pretreatment, Enzymatic Hydrolysis & Fermentation
Deep Eutectic Solvents
Enzyme stability in deep eutectic solvents
Ionic Liquids Applications
Project title: Development of Future Antibiotics: Active Pharmaceutical Ingredient Ionic Liquids (API-ILs) with COSMO-RS Based Prediction for Resistant Microbial strains.